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Standard dosage: 200–400 mg silymarin daily, divided into 2–3 doses Ideally taken before meals For liver stress: up to 420 mg silymarin daily may be appropriate — only after medical consultation Alcohol should be avoided during intake ⚠️ Milk thistle preparations during pregnancy and breastfeeding should only be taken on the explicit recommendation of a doctor or midwife

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The expression of eNOS and COX-2 mRNA was normalized to the reference gene GAPDH mRNA expression